Abstract—Twenty four HFxDeoni cross bred cows were divided into four groups (6 cows in each group) on the basis of average milk yield, parity and stage of lactation. T₀ (control group) cows were not fed with probiotics. T₁, T₂ and T₃ (treatment groups) cows were fed with 10 gm, 15 gm and 20 gm probiotics per day, respectively, just before morning milking. The multi strain probiotic contained Saccharomyces cervisiae and Lactobacillus sporogenes. The animals were milked twice in a day; morning at 5.30 am and afternoon 3.30 pm. Daily milk yield was recorded in pre-trial period of 25 days and then during 60 days of experimental period. Milk samples from the individual cows were collected twice a week (in pre-trial period and in experimental period) and were analysed for fat, SNF, density, freezing point, protein, lactose and total ash using the milk analyzer. From pre-trial period to trial period, the milk yield increased from 8.31 L/day, 8.26 L/day and 8.48 L/day to 8.97L/day, 9.64L/day and 9.68L/day in T₁, T₂ and T₃ group (highly significant; P<0.05), respectively, compared to from 8.45 L/day to 8.57L/day in T0 group. Milk were significantly higher in cows (T₁, T₂ and T₃) supplemented with probiotics than T₀. The freezing point decreased in T₁, T₂ and T₃ groups indicating increase in the total solids of milk compared to T0. There were minor changes in ash content of milk by feeding probiotics. Economically, supplementing the diet with probiotic earned more profit and feeding @ 15 gm probiotic/day/animal was found more beneficial than feeding @ 10 and 20 g/day/cow.

Index Terms—probiotic, saccharomyces cervisiae, lactobacillus sporogenes, milk yield, milk composition and economics

Cite: J. N. Shreedhar, Manjunath Patil, and Pradeep Kumar, "Effect of Probiotics Supplementation on Milk Yield and Its Composition in Lactating Holstein Fresien and Deoni Cross Bred Cows," Journal of Medical and Bioengineering, Vol. 5, No. 1, pp. 19-23, February 2016. Doi: 10.12720/jomb.5.1.19-23